Tuesday, May 5, 2009

interview

Tarek Edelbi
Interview
I chose Dr. Raymond Otto as the subject for my interview. He is the new assistant professor of genetics in the Department of Molecular and Microbiology at George Mason University. Professor Otto is currently my Genetics 311 teacher as well. Throughout the course of the interview I was able to learn a lot about Dr. Otto’s career from his time as an undergraduate student up until now. For both his undergraduate and graduate years he attended the University of North Carolina at Chapel Hill. In his undergraduate years he double majored in zoology and chemistry and as for his graduate years he majored in doctorial genetics.
Although he has dedicated about twenty years of his life to research under the subtopic of gene therapy, he has spent the last three years teaching. He spends approximately 90% of his time to teaching and 10% of his time conducting committee work. Dr. Otto interacts with hundreds of students daily and they change from semester to semester. He has the opportunity to familiarize himself with a multitude of personalities. One aspect of the student’s attitudes that surprised professor Otto was a significant sense of complacency. The Professor had expected students to rise to the challenge of more rigorous coursework. I then geared the interview questions towards my research topic, how chemicals in plastics may alter DNA or cell structure.
I raised the question to see if he may have had any prior experience or knowledge on the topic. He was aware of many of the general mutagens and stated that he has worked with viral safety and not so much with the chemical safety aspect. I proceeded to ask him if he has done any general research related to the topic. Although he has been involved in numerous significant research projects and initiatives, he hasn’t really touched this particular topic. I asked where he would start researching the topic, should he want to. He went on to say that he would need to do a lot of preliminary research on the subject, to familiarize himself with the topic and be able to assess what aspects of the problem to investigate first. While not being familiar with chemicals contained in plastics that cause mutations he stated that he is very knowledgeable on the various methods he would use to conduct his research, since he has done chemical testing on drugs and small molecule drugs.
When I asked him if this topic is a growing concern in genetics, he responded saying that although research is being conducted on the subject it is more of a growing concern to for the consumers than it is to geneticists. I went on to question him about further research he has been involved in. Dr. Otto is more attracted toward gene structure and regulation and how fluid it is. He discussed briefly in his previous work how he had cloned not only drosophila but also human genes. He has also worked in the field of cancer research and mutations in the genome. He has also been involved in FDA work for UNC in regulatory aspects of emerging technology. Dr. Otto has worked on everything from specialized research to the public safety policy on emerging technology.
When asked if there is anything else he would like to add to the interview he acknowledged the fact that most of the students he teaches are in the pre-medical or pre-dental field and haven’t even considered entering the emerging field of biotechnologies. He explained that a few students seek positions in this field as undergraduates. It’s a great opportunity since we are located in one of the countries four biotechnology hub. This interview went very well over all and I was a great experience.

interview

Tarek Edelbi
Interview
I chose Dr. Raymond Otto as the subject for my interview. He is the new assistant professor of genetics in the Department of Molecular and Microbiology at George Mason University. Professor Otto is currently my Genetics 311 teacher as well. Throughout the course of the interview I was able to learn a lot about Dr. Otto’s career from his time as an undergraduate student up until now. For both his undergraduate and graduate years he attended the University of North Carolina at Chapel Hill. In his undergraduate years he double majored in zoology and chemistry and as for his graduate years he majored in doctorial genetics.
Although he has dedicated about twenty years of his life to research under the subtopic of gene therapy, he has spent the last three years teaching. He spends approximately 90% of his time to teaching and 10% of his time conducting committee work. Dr. Otto interacts with hundreds of students daily and they change from semester to semester. He has the opportunity to familiarize himself with a multitude of personalities. One aspect of the student’s attitudes that surprised professor Otto was a significant sense of complacency. The Professor had expected students to rise to the challenge of more rigorous coursework. I then geared the interview questions towards my research topic, how chemicals in plastics may alter DNA or cell structure.
I raised the question to see if he may have had any prior experience or knowledge on the topic. He was aware of many of the general mutagens and stated that he has worked with viral safety and not so much with the chemical safety aspect. I proceeded to ask him if he has done any general research related to the topic. Although he has been involved in numerous significant research projects and initiatives, he hasn’t really touched this particular topic. I asked where he would start researching the topic, should he want to. He went on to say that he would need to do a lot of preliminary research on the subject, to familiarize himself with the topic and be able to assess what aspects of the problem to investigate first. While not being familiar with chemicals contained in plastics that cause mutations he stated that he is very knowledgeable on the various methods he would use to conduct his research, since he has done chemical testing on drugs and small molecule drugs.
When I asked him if this topic is a growing concern in genetics, he responded saying that although research is being conducted on the subject it is more of a growing concern to for the consumers than it is to geneticists. I went on to question him about further research he has been involved in. Dr. Otto is more attracted toward gene structure and regulation and how fluid it is. He discussed briefly in his previous work how he had cloned not only drosophila but also human genes. He has also worked in the field of cancer research and mutations in the genome. He has also been involved in FDA work for UNC in regulatory aspects of emerging technology. Dr. Otto has worked on everything from specialized research to the public safety policy on emerging technology.
When asked if there is anything else he would like to add to the interview he acknowledged the fact that most of the students he teaches are in the pre-medical or pre-dental field and haven’t even considered entering the emerging field of biotechnologies. He explained that a few students seek positions in this field as undergraduates. It’s a great opportunity since we are located in one of the countries four biotechnology hub. This interview went very well over all and I was a great experience.

Tuesday, April 14, 2009

scientists in the schools

Scientists in the Schools
Learning objectives:
The learning objective of the program is to help the students have a better understanding of how chemicals and toxins leach into plastics and alter genetic compositions of cells. The main goal of the program is for the students to gain enough of an understanding of the subject to maintain discussions on recycling and the importance of finding alternatives to plastics.
Lesson Plan:
The target age range for the students is 16-17 year old high school students. This age range was chosen because the students have basic knowledge of science but are still unaware of any of the specifics of this particular field of research. This makes them perfect candidates for a learning based environment because they are able to comprehend the basic subject matter of the work and are interested because of their relative lack of knowledge.
The program will take place in a university setting as it will be an appropriate environment for college level work. The atmosphere is meant to encourage the students to do better and take the work more seriously. The class will be held three times a week on Monday, Wednesday and Friday for a length of one and half hours per session. The schedule will also accommodate these up and coming students to a college setting. The program will run for a length of two weeks, making the course less intimidating and keeping the students from becoming too discouraged or overwhelmed.
The actual course will have three major parts. In class lectures, labs and online coursework. The first of these components, lectures, is meant to give the students a base understanding of the background on the research topic. The students will learn about how cells and their genetic materials can be altered. This topic has a few different branches and subtopics so it will be taught over the two week period. The lectures are absolutely essential in giving students an understanding of the material.
The latter two parts of the course are meant to be supplemental and finalize the students understanding. The in class labs will visually illustrate how plastics are made and how they can be recycled. Giving students this type of tangible hands on knowledge about plastics will be quite effective and ensure that the lessons taught in the lecture are reinforced and enhanced with new information.
The last part of the course will be student’s online coursework. This assignment will allow the students the flexibility to complete these assignments at their own leisure. The nature of the assignments will be closely tied to the lecture and lab coursework. For example, one of the later assignments is to write a short report suggesting a possible solution to the problems plastics present in the environment. The responses will be highly variable and the purpose of the assignment is to tap into the students’ creativity and generate interest in the subject.
Assesement:
The effectiveness of the course will be assessed by beginning examinations of students’ knowledge of the subject matter and an ending evaluation of their knowledge plus a student evaluation response to the program itself. The main purpose of the last student evaluation of the course is to provide the program with information they need to enhance the program or alter certain aspects to make it more effective and efficient.

Monday, March 23, 2009

summary II for 3/24/09

The second article I read was about a reemerging research about cold fusion, which is stirring up more interest. After 20 years of debating cold fusion is receiving a new impetus at the American Chemical Society's national meeting in the US this week. Cold fusion was basiclly claimed to be a boundless source of clean energy by Martin Fleischmann and Stanley Pons. There have been a numerous failed attempts to replicate thier experiments but none succesful although researchers insist that cold fusion is possible. With the world facing an energy crisis, it is well worth exploring all possibilities. The principle of cold fusion runs counter to that of other fusion production mechanisms that employ enormous lasers or magnetic chambers to contain searingly hot gas. Pons and Fleischmann experiment was conducted by running a current through a room-temperature device called an electrolytic cell. As the heat rised in the cell which suggested that power was being produced within it from nuclear fusion. However Pons and Fleischmann couldnt establish that cold fusion is a reality.

summary for 3/24/09

The article i read was titled New DNA test gives cold case hope. I found it on the BBC news website. it describes how a new technique which can decipher previously unintelligible DNA samples, that where destroyed on scene, has been made available to all police forces in England and Wales. This is a huge break through for the Forensic Science Service in england and wales because these new methods can now lead to thousands of unsolved cases. Scientists can now obtain individual DNA from crime scenes which contain a mix of genetic material from several different people. The Forensic Science Service said it helped police identify suspects and build evidence for cases. Previously, it was impossible for scientists to accurately separate out DNA from different individuals at one crime scene, an example given in the article was where two or three suspects handled the same weapon. Now with the new DNAboost computer technology can do just that and provide identifiable DNA profiles for each suspect, which can be checked against the national DNA database to try to find the culprit. Experts say it could increase the number of provable cases by as much as 30%.

Thursday, March 19, 2009

literature review blog

1) Is it giving information and comparing different research or is it more persuasive?
2) Does the paper seem repetitive?
3) Is more research needed?
4) Does the paper flow well?
5) is the paper to "wordy"?

literature review

Tarek Edelbi
Literature Review

Plastic is one of the most heavily used materials in present day society. It’s present in the food we eat, the water we drink, and many of our material possessions. As a result of our constant interaction with the material it is also present in the air we breathe, and will soon start to take a negative toll on organic life forms. Plastics are made of non-biodegradable materials although through various obstacles such as the passage of time, photodegradation, and ultraviolet rays from the sun, plastic can break down and create chemical byproducts over a period of time. Over 400 billion pounds of plastic is being produced every year exceeding the previous year’s production. These byproducts can adversely affect the living conditions on earth. Plastic in a sense never ceases to exist because it can change form. Numerous chemical by-products and toxins are released, not only through the break down but from various plastic products such as hair combs, water bottles, toys etc.
For the basics plastic is made by combining many toxic synthetic man-made chemicals by a process called polymerization. This process technicilly binds the toxic chemicals together so tightly that they are no longer toxic to organisims. Although the polymerization process will most likely never be flauless, it always leaves various toxic chemicals sustainable to transfer out of the plastic material and into its contacts. Toxins and chemicals released from these plastics can be extremely detrimental to all living life forms. Some toxins and chemicals in plastics known to be detrimental are bisophenol A, antimony, and Phthalates.
These toxins are known to cause different forms of cancer-causing cells and also are hormone altering and imitating. On a much larger scale they have the potential to affect DNA as well as the genetic make-up. Since cancer is more prevalently studied, it attracts more researchers, and therefore more recent studies are conducted on the cancer causing aspects of plastics. A recent study conducted by the American Association for Cancer Research showed that chemicals such as bisophenol A in plastics will stimulate the growth of various cancer-causing cells which may lead to mammary and prostate cancer.
Bisophenol A or BPA is generally found in heavier plastic packaging, such as those used for milk storage, container linings and water supply pipes. A recents study done by a coalition of environmental health NGOs stated that BPA which is also a synthetic sex hormone which can mimics estrogen is used to make hard polycarbonate plastic. On average these plastics release between 4.7 – 8.3 parts per billion of bisphenol A. A recent research on animals showed that bisphenol A can be harmful by disrupting development at doses even below these levels. Other research state that although chemical is prone to affect an organism at any stage of its life, the toxins are more effective at key stages of development and may be life-altering even in minus increments.
Another toxin such as antimony is also known to be carcinogenic. One study done in long term exposure with only minimal amounts of antimony yielded with organisms such as animals in this case developed eye irritation, hair loss, lung damage, and heart problems. Another study composed, only now the organisms were exposed to higher levels of antimony, the animal developed fertility problems as well as lung cancer. Chemicals such as Phthalates are also an additive in plastic which helps the flow of molecules in the material to give texture and flexibility. A research, done by the Centers for Disease Control and Prevention on humans, shows metabolites of multiple phthalates in urine, which is believed to be due to plastics. Baby care products are a growing concern due to the fact that most of them contain phthalates. The study also stated that young infants are more vulnerable to the potential effects of phthalates due to their increased dosage per unit body surface area, metabolic capabilities, and developing endocrine and reproductive systems.
A study was also done on rodents which were exposed to certain phthalates levels. In the case of high doses a change in hormone levels was evident and birth defects were observed. One explained that human phthalate exposure during pregnancy resulted in decreased anogenital distance among baby boys. In this study, phthalate metabolites were measured in urine samples collected from the pregnant women who gave birth to the infants. After birth, the genital features and anogenital distance of these women's babies were measured and correlated with the residue levels in the mother's urine. Boys born to mothers with the highest levels of phthalates were seven times more likely to have a shortened anogenital distance.
A research conducted by the American government journal Environment Health Perspectives stated that BPA and phthalates were gaining a reputation for being endocrine disrupters. Chemicals from plastic even have the frightening potential to change DNA or genetic make-up.




Work Cited
Ahmad, Maqbool, and Ahmad S. Bajahlan. "Leaching of styrene and other aromatic compounds in drinking water from PS bottles." 16 May 2007. Science Direct. Journal of environmental sciences. Mason, Fairfax. 25 Feb. 2009 .
Alderson, Norris. "Testimony." 14 Mar. 2008. ASL. Mason, Fairfax. 25 Feb. 2009 .
Ball, Douglas. "Development of Safety Qualification Thresholds and Their Use in Orally Inhaled and Nasal Drug Product Evaluation." 23 Feb. 2007. Toxicological Sciences. ToxSci Advance Access. Mason, Fairfax. 25 Feb. 2009 .
"Screening Assessment for The Challenge Phenol, 4,4' -(1-methylethylidene)bis- (Bisphenol A) Chemical Abstracts Service Registry Number 80-05-7." Oct. 2008. Environment Canada. Health Canada. Mason, Fairfax. 25 Feb. 2009 .
C.J. Moore et al., (2001) “A Comparison of Plastic and Plankton in the Pacific Central Gyre,” Marine Pollution Bulletin 42: 297-1300. The North Pacific Gyre and the ORV Alguita Somorovská, M., J. Tulinská, M. BaranÄoková, M. ZámeÄnÃková, A. Collins, A. LÃÅ¡ková, B. Vallová, H. Petrovská, E. Jáhnová, P. VodiÄka, L. Fuortes, and M. DuÅ¡inská. "THE COMET ASSAY IN BIOMONITORING OF OCCUPATIONAL EXPOSURE IN RUBBER FACTORY AND PLASTIC LAMINATION PLANT. COMPARISON WITH CYTOGENETIC AND IMMUNE BIOMARKERS." 2006. ELIS. MASON, Fairfax. 25 Feb. 2009 .
Totten, Bill. "[A-List] Get Plastic Out of Your Diet." 16 Nov. 2006. Mindfully. Livivng nutrition magazine. Mason, Fairfax. 25 Feb. 2009 .
Tyl, R. "Polycarbonate Plastics and Bisphenol A Release." 2006. Bisphenol A. Human health and saftey. Mason, Fairfax. 25 Feb. 2009
Vom Saal, F. S., W.V. Welshons, & S. Parmigiani “Leaching of Bisphenol A From Polycarbonate Plastic Disrupts Development via Epigenetic Mechanisms.” Prepared for the Erice International Seminars on Planetary Emergencies, Erice, Italy. 19-26 August 2006.